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KMID : 0903519980410060410
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1998 Volume.41 No. 6 p.410 ~ p.416
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Purification of a major protein with physiological activities from Panax ginseng C. A. Meyer
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박훈/Park, H .
오세량/김경현/권택헌/Oh, Sei R ./Kim, Kyung H ./Kwon, Taek H .
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Abstract
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The major protein (GMP) from the roots of Panax ginseng C. A. Meyer was purified, using gel filtration and ion exchange chromatography followed by reversed-phase and ion exchange FPLC. Staining analysis indicated that the protein has a carbohydrate moiety, which was also shown by band shift experiments using various glycosidases. Electrophoretic and gel permeation studies showed that GMP has an apparent molecular weight of 63 kDa composed of possibly two subunits of 25 kDa containing carbohydrate moiety. GMP showed an anticomplementary activity on the hemolysis of red blood cells, which is a screening tool for inflammation mediator search.
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